Skip to main content

News

Design of new protein scaffolds from multiple chains and their regulation

Lapenta et al., Nat.Comm. 2021.

Proteins are key building blocks of all organisms. In recent years, we realized that new protein structures can be constructed based on different architecture than natural proteins. One such example is protein origami from coiled-coils. In this case, the edges of self-assembling protein cages consist of designed coiled coil dimers that are connected into a single polypeptide chain. In this way we designed a tetrahedron, a pyramid and a trigonal prism. Assembly from several polypeptide chains would enable the design of more complex shapes since we could use the same building blocks in different chains. This advance was reported by a team at the National Institute of Chemistry that showed that they can assemble a protein bipyramid from two polypeptide chains in several ways. In addition to designing a new protein fold, they also designed a way to regulate the assembly by an enzyme acting as a trigger. This research paves the way towards the design of more complex structures, which could be used for delivery and release of drugs, catalysis and for vaccines.

The research funded by the ERC AdG project MaCChines led by Roman Jerala, head of the Department of Synthetic Biology at the Institute of Chemistry and Slovenian Research Agency was published in the journal Nature Communications. The authors of the research are Fabio Lapenta, Jana Aupič, Marco Vezzoli, Žiga Strmšek, Stefano Da Vela, Dmitri I. Svergun, José María Carazo, Roberto Melero and Roman Jerala.

https://www.nature.com/articles/s41467-021-21184-6

The discovery of the CRISPR/Cas system was awarded the 2020 Nobel Prize in Chemistry in 2020. Researchers from the…
Ljubljana, 10 November 2021 - Researchers from the Department of Inorganic Chemistry and Technology (D09), Prof. Dr.…
Ljubljana, July 6, 2021 - The Corona crisis has been plaguing us for almost a year and a half. It has posed…
Ljubljana, January 7th 2021 - Assoc. Prof. Dr. Nejc Hodnik, researcher at The National Institute of Chemistry and the…
Ljubljana, 31 August 2020 - At the National Institute of Chemistry, we prepared a press conference to present the…
Accessibility(CTRL+F2)
color contrast
text size
highlighting content
zoom in