Development of biological tests and measurements of in vitro biological activity of (recombinant) proteins | |
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Laboratory | D11 |
Equipment | BSL2 laboratory for cell biology (2 LAFs, 2 CO2 incubators, multimodular plate reader, multi-mode reagent dispenser, invert microscope), multi-modular liquid N2 culture storage facility, fluorimeter, confocal microscope (D12). |
Field | Biological and biochemical studies |
Platform | Analysis of biological samples |
Technics | Development and qualification of the method, measurements of in vitro biological activity on cell cultures, hemolysis, calcein release experiments |
Contact | izr. prof. dr. Marjetka Podobnik marjetka.podobnik(at)ki.si +386 1 4760 372 |
Description | Establishment of in vitro biological tests and measurements of protein activity on cell cultures, determination of the pore-forming activity of proteins on erythrocytes (hemolysis) or artificial lipid vesicles by monitoring the release of calcein or the intrusion / release of other fluorescent molecules.
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