The PEAQ-ITC isothermal titration calorimeter (MicroCal, Malvern Panalytical) enables the study of molecular interactions involving proteins, antibodies, nucleic acids, small molecules, and other biomolecules. By directly measuring the heat released or absorbed during an interaction, it allows for the determination of binding affinity, stoichiometry, and thermodynamic parameters—such as enthalpy and entropy. It is used for characterizing molecular interactions, screening and optimizing target compounds, designing inhibitors, and studying enzyme kinetics. Measurements can be performed without the need for labeling or immobilizing the molecules.
The equipment is available to internal and external users upon prior inquiry. Interested users should contact the person in charge of the instrument. Depending on prior arrangements, the equipment may be used by trained operators, or measurements can be conducted by staff from the Department of Molecular Biology and Nanobiotechnology.
