Research equipment
Experimental and technical expertise and equipment
We use a wide range of interdisciplinary methodology including biophysical techniques (DLS, MALS, CD, fluorescence, luminescence, flow cytometry, confocal fluorescence microscopy, FCS), molecular biology and biochemistry (DNA cloning, molecular modeling, protein design, CRISPR/Cas9) to animal experiments (in-house animal facility, transgenic and KO mice, implantation of encapsulated cellular devices, study of pathophysiology).
We recently obtained a cryo-electron microscope and SAXS system, which will be particularly important for the research of designed protein cages but also for immunology.
List of equipment
- Cryo electron microscope Glacios
- LUMICKS C-Trap EDGE 350: TIRF @ 488nm, 532 nm and 638 nm, IRM, optical tweezers with single molecule force measurement and a six-channel microfluidic system
- CX7 Cell Insight: automated time-lapse monitoring of fluorescence, colour and transmitted light imaging of cells
- Small angle X-ray scattering measurment system SAXSpoint 5.0 System Anton Paar with Primux 100 micro X-ray source (Cu)
- Spectral flow cytometer CyTek Aurora
- Microscope for spatial and temporal imaging of life cells Leica SP8: imaging of spatial and temporal changes in fluorescence-labelled molecules in cells
- Confocal microscope Leica SP5
- Chromatographic system for protein purification Äkta (3 systems) with programmable injector and fraction collector
- Static light scattering (SLS) detector Wyatt Dawn Heleos 8+
- LC-quadrupole mass spectrometer Shimadzu
- HPLC chromatograph with manual injector and fraction collector: separation of proteins and peptides
- IVC Techniplast Equipment: laboratory animal breeding and performing in vivo experiments
- Circular dichroism (CD) spectrometer Applied Photophysics Chirascan
- Fluorescent spectrometer Perkin Elmer LS 55
- Plate readers for detection of luminescence, fluorescence or absorbance: Berthold Mithras, Berthold LB 963 Centro, BioTek SynergyMx
- Automated workstation for precise automatic pipetting, Perkin Elmer Janus
- Equipment for particle size and zeta potential Malvern
- Stopflow Biologic SFM-3000 with MOS-200 spectrophotometer
- Biorad Gene Pulser Xcell electroporator: to facilitate entry of plasmid DNA into pro- and eucariont cells
- Ultrasound generating system Verasonics UTA 260-S
- Bioimager IVIS Lumina LT Inst: detection of luminescence in vivo
- Ultra Centrifuge Beckman Optima L 100 XP
Department is hosting Centre for biospectroscopy OE2.
Access is possible upon appointment and consultation. Please write to martina(dot)mohorcic(at)ki(dot)si