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Research equipment

Experimental and technical expertise and equipment

We use a wide range of interdisciplinary methodology including biophysical techniques (DLS, MALS, CD, fluorescence, luminescence, flow cytometry, confocal fluorescence microscopy, FCS), molecular biology and biochemistry (DNA cloning, molecular modeling, protein design, CRISPR/Cas9) to animal experiments (in-house animal facility, transgenic and KO mice, implantation of encapsulated cellular devices, study of pathophysiology).

We recently obtained a cryo-electron microscope and SAXS system, which will be particularly important for the research of designed protein cages but also for immunology.

List of equipment

  • Cryo electron microscope Glacios
  • LUMICKS C-Trap EDGE 350: TIRF @ 488nm, 532 nm and 638 nm, IRM, optical tweezers with single molecule force measurement and a six-channel microfluidic system
  • CX7 Cell Insight: automated time-lapse monitoring of fluorescence, colour and transmitted light imaging of cells
  • Small angle X-ray scattering measurment system SAXSpoint 5.0 System Anton Paar with Primux 100 micro X-ray source (Cu)
  • Spectral flow cytometer CyTek Aurora
  • Microscope for spatial and temporal imaging of life cells Leica SP8: imaging of spatial and temporal changes in fluorescence-labelled molecules in cells
  • Confocal microscope Leica SP5
  • Chromatographic system for protein purification Äkta (3 systems) with programmable injector and fraction collector
  • Static light scattering (SLS) detector Wyatt Dawn Heleos 8+
  • LC-quadrupole mass spectrometer Shimadzu
  • HPLC chromatograph with manual injector and fraction collector: separation of proteins and peptides
  • IVC Techniplast Equipment: laboratory animal breeding and performing in vivo experiments
  • Circular dichroism (CD) spectrometer Applied Photophysics Chirascan
  • Fluorescent spectrometer Perkin Elmer LS 55
  • Plate readers for detection of luminescence, fluorescence or absorbance: Berthold Mithras, Berthold LB 963 Centro, BioTek SynergyMx
  • Automated workstation for precise automatic pipetting, Perkin Elmer Janus
  • Equipment for particle size and zeta potential Malvern
  • Stopflow Biologic SFM-3000 with MOS-200 spectrophotometer
  • Biorad Gene Pulser Xcell electroporator: to facilitate entry of plasmid DNA into pro- and eucariont cells
  • Ultrasound generating system Verasonics UTA 260-S
  • Bioimager IVIS Lumina LT Inst: detection of luminescence in vivo
  • Ultra Centrifuge Beckman Optima L 100 XP

Department is hosting Centre for biospectroscopy OE2.

Access is possible upon appointment and consultation. Please write to martina(dot)mohorcic(at)ki(dot)si

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